The “dark matter” of the genome—non-coding DNA—constitutes over 98% of our genetic material, yet its significance has only begun to be uncovered. Non-coding DNA gets its name because it does not code for proteins. However, these non-coding regions contain critical regulatory elements, such as non-coding RNAs, that influence gene expression and impact vital cellular processes. Studying this genomic “dark matter” is essential for understanding complex biological functions, from development to disease, and could lead to breakthroughs in treating conditions like cancer, neurodegenerative diseases, and aging.
My work has primarily focused on uncovering new roles for microRNAs in Caenorhabditis elegans. MicroRNAs are small, ~22-nt, non-coding RNAs that play important roles in regulating post-transcriptional gene-expression. It’s an exciting time to be microRNA enthusiast as the discovery of these small, non-coding RNAs was recently recognized with the 2024 Nobel Prize!
To investigate these non-coding RNAs, I use the model organism C. elegans. These tiny nematodes share similar types of non-coding RNAs with humans, making them ideal for genetic studies. By combining genetic, molecular biology, and bioinformatic techniques, my research has revealed new roles for non-coding RNAs as key regulators of stress and aging. In this relatively new field, much is left to be discovered!
Publications
Chipman LB, Luc S, Nicastro IA, Hulahan JJ, Dann DC, Bodas DM, Pasquinelli AE. Expression, not sequence, distinguishes miR-238 from its miR-239ab sister miRNAs in promoting longevity in Caenorhabditis elegans. PLoS Genet. 2023 Nov 27;19(11):e1011055. doi: 10.1371/journal.pgen.1011055. PMID: 38011256; PMCID: PMC10703411.
Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans
Pagliuso DC, Bodas DM, Pasquinelli AE. Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans. PLoS Genet. 2021 Aug 5;17(8):e1009734. doi: 10.1371/journal.pgen.1009734. PMID: 34351906; PMCID: PMC8370650.
Remodeling of the Caenorhabditis elegans non-coding RNA transcriptome by heat shock
Schreiner WP, Pagliuso DC, Garrigues JM, Chen JS, Aalto AP, Pasquinelli AE. Remodeling of the Caenorhabditis elegans non-coding RNA transcriptome by heat shock. Nucleic Acids Res. 2019 Oct 10;47(18):9829-9841. doi: 10.1093/nar/gkz693. PMID: 31396626; PMCID: PMC6765114.
Additional Scholarly Activity
In addition to scientific research, I am interested in supporting equitable access to science education and advancement for all students. Through careful needs assessments and strategic partnerships, I have developed an array of programming to advance these initiatives. One area I am particularly passionate about is mentorship.
Check out my article published in Inside Higher Ed to learn about one of these programs which offers a model for alumni mentorship: https://www.insidehighered.com/opinion/career-advice/carpe-careers/2024/09/30/how-alumni-can-help-guide-biomedical-trainees-their
Dr. Delaney Dann 